High RNA quality extracted from the tolerant crop Cyamopsis tetragonoloba (L.) despite possession of low RNA integrity number
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چکیده
منابع مشابه
RNA Integrity Number (RIN) – Standardization of RNA Quality Control Application
The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Using intact RNA is a key element for successful microarray or RT-PCR analyses. The Agilent 2100 bioanalyzer and RNA LabChip kits play an important role in assisting researchers in the determination of RNA quality. Profiles generated on the Agilent 2100 bioanalyzer yield information on concent...
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The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as...
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ژورنال
عنوان ژورنال: Biotechnology & Biotechnological Equipment
سال: 2021
ISSN: 1310-2818,1314-3530
DOI: 10.1080/13102818.2021.1910567